Activated endothelial cells induce neutrophil extracellular traps and are susceptible to NETosis-mediated cell death
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1194057
Author(s) Gupta, Anurag Kumar; Joshi, Manjunath B; Philippova, Maria; Erne, Paul; Hasler, Paul; Hahn, Sinuhe; Resink, Therese J
Author(s) at UniBasel Erne, Paul
Resink, Thérèse J.
Year 2010
Title Activated endothelial cells induce neutrophil extracellular traps and are susceptible to NETosis-mediated cell death
Journal FEBS letters
Volume 584
Number 14
Pages / Article-Number 3193-7
Keywords Neutrophil, Neutrophil extracellular trap, Endothelial cell
Abstract Neutrophil interaction with activated endothelial cells (EC) is required for transmigration. We examined consequences of this interaction on NETosis. Co-culture of activated EC with neutrophils induced neutrophil extracellular trap (NET) formation, which was partially dependent on production of IL-8 by activated EC. Extended neutophil/EC co-culture resulted in EC damage, which could be abrogated by inclusion of either diphenyleneiodonium to inhibit the NAPDH oxidase pathway required for NETosis, or DNAse to disrupt NETs. These findings offer new insight into mechanisms whereby NETs trigger damage to the endothelium in sepsis, small vessel vasculitis and possibly the villous trophoblast in preeclampsia.
Publisher Elsevier Science
ISSN/ISBN 0014-5793
edoc-URL http://edoc.unibas.ch/dok/A6004289
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.febslet.2010.06.006
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/20541553
ISI-Number WOS:000279438800038
Document type (ISI) Journal Article
 
   

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