Contractile cell forces exerted on rigid substrates

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Contractile cell forces exerted on rigid substrates

JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)

ID	1193860
Author(s)	Köser, Joachim; Gaiser, Sebastian; Müller, Bert
Author(s) at UniBasel	Müller, Bert
Year	2011
Title	Contractile cell forces exerted on rigid substrates
Journal	European cells & materials
Volume	21
Pages / Article-Number	479-86; discussion 486-7
Keywords	Cell contractility, rat-2 fibroblast, in vitro test, atomic force microscopy, biosensor
Abstract	Adhesive cells including fibroblasts produce contractile forces to the underlying substrate for their locomotion. Such forces are not only high enough to deform compliant membranes such as silicone but also bend rigid micro-cantilevers. The cell-induced bending of silicon micro-cantilevers was determined using laser deflection during trypsin treatment. The observed cantilever relaxation corresponds to a contractile cell force of (16±7) µN per rat-2 fibroblast. The cantilever bending approach represents a unique method for the determination of contractile cell forces on any kind of rigid substrate in desired (physiological) environment. Hence, the fundamental technique allows building cell-based biosensors or should provide a quantitative parameter for characterising the cyto-compatibility of load bearing implant surfaces.
Publisher	Univ. of Wales
ISSN/ISBN	1473-2262
edoc-URL	http://edoc.unibas.ch/dok/A6004098
Full Text on edoc	No
PubMed ID	http://www.ncbi.nlm.nih.gov/pubmed/21623572
ISI-Number	WOS:000311234100006
Document type (ISI)	Journal Article

25/04/2024

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