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A comparison between ((3)H)-thymidine incorporation and isothermal microcalorimetry for the assessment of antigen-induced lymphocyte proliferation
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1193648
Author(s) Murigande, C; Regenass, S; Wirz, D; Daniels, A U; Tyndall, A
Author(s) at UniBasel Daniels, A.U. Dan
De Vere-Tyndall, Alan
Year 2009
Title A comparison between ((3)H)-thymidine incorporation and isothermal microcalorimetry for the assessment of antigen-induced lymphocyte proliferation
Journal Immunological investigations
Volume 38
Number 1
Pages / Article-Number 67-75
Keywords Microcalorimetry, Lymphocyte proliferation, Antigen stimulation
Abstract Lymphocyte transformation tests (LTT) are time-consuming radioactive assays used in the clinic for the determination of allergic drug reactions and extensively in basic immunological research. In the present study we propose an alternative method in the monitoring of T-cell responses by isothermal microcalorimetric (IMC) measurements of overall cellular heat production as a function of time. For mitogen-induced lymphocyte proliferation, we analyzed a concentration dependent effect of phytohemaglutinin (PHA) and both tests showed a good correlation. This was also the case for specific antigenic stimulation with Varidase(R) or tetanus toxoid. On the other hand, antigen-induced lymphocyte proliferation analyzed by pre and post influenza vaccine (Inflexal(R) V) samples, showed no such correlation. Our study suggests that IMC measurements, despite the advantages of simplicity, on-line recording of metabolic activity and no use of radioactivity, may be limited to monitoring mitogen-induced lymphocyte proliferation.
Publisher Taylor & Francis
ISSN/ISBN 1532-4311
edoc-URL http://edoc.unibas.ch/dok/A6003887
Full Text on edoc No
Digital Object Identifier DOI 10.1080/08820130802572160
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/19172486
ISI-Number WOS:000262788500005
Document type (ISI) Journal Article
 
   

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