A novel three-dimensional culture system allows prolonged culture of functional human granulosa cells and mimics the ovarian environment
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1192675
Author(s) Kossowska-Tomaszczuk, Katarzyna; Pelczar, Pawel; Güven, Sinan; Kowalski, Jacek; Volpi, Emanuela; De Geyter, Christian; Scherberich, Arnaud
Author(s) at UniBasel de Geyter, Christian M.H.R.
Scherberich, Arnaud
Year 2010
Title A novel three-dimensional culture system allows prolonged culture of functional human granulosa cells and mimics the ovarian environment
Journal Tissue Engineering
Volume 16
Number 6
Pages / Article-Number 2063-2073
Abstract The development of techniques allowing the growth of primordial follicles to mature follicles in vitro has much potential for both reproductive medicine and developmental research. However, human primordial and preantral follicles fail to grow after isolation from the surrounding ovarian stroma. Granulosa cells, which normally undergo apoptosis after ovulation, contain a subpopulation of ovarian follicular cells remaining viable in vitro over prolonged periods when cultured in the presence of leukemia-inhibiting factor. However, when cultured as monolayers, they progressively lose all their characteristics, such as follicle-stimulating hormone receptor and cytochrome P450-aromatase. Here, we describe a three-dimensional culture system containing type I collagen, which, together with leukemia-inhibiting factor, allowed the survival and growth of a subpopulation of granulosa cells isolated from mature ovarian follicles and supported them to proliferate into spherical structures exhibiting steroidogenic capacity, as demonstrated by P450-aromatase and 3beta-hydroxysteroid dehydrogenase. After transplantation into the ovaries of immunodeficient mice, these cells became localized preferentially within antral follicles and the prolonged expression of follicle-stimulating hormone receptor was confirmed as well. With this optimization of the culture conditions, an environment was created, which acts as a niche closely mimicking the development of early ovarian follicles in vitro.
Publisher Mary Ann Liebert
ISSN/ISBN 1937-3376 ; 1937-335X
edoc-URL http://edoc.unibas.ch/dok/A6002925
Full Text on edoc No
Digital Object Identifier DOI 10.1089/ten.TEA.2009.0684
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/20109057
ISI-Number WOS:000278164800024
Document type (ISI) Article
 
   

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