4D solid-state NMR for protein structure determination
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 1171035
Author(s) Huber, Matthias; Böckmann, Anja; Hiller, Sebastian; Meier, Beat H.
Author(s) at UniBasel Hiller Odermatt, Sebastian
Year 2012
Title 4D solid-state NMR for protein structure determination
Journal Physical Chemistry, Chemical Physics
Volume 14
Number 15
Pages / Article-Number 5239-46
Abstract Solid-state NMR offers the chance to extend structural studies to proteins that are otherwise difficult to study at atomic resolution, such as protein fibrils, membrane proteins or poorly diffracting crystals. As two-dimensional spatial correlation NMR spectra of proteins suffer from severe resonance overlap, we analyze in this perspective article the potential of higher-dimensional (3D and 4D) proton-detected experiments, which have an increased number of identifiable and assignable distance restraints for solid-state structural studies. We discuss practical considerations for the NMR measurements and the preparation of suitable protein samples and show results of structure calculations from 4D solid-state NMR spectra.
Publisher Royal Society of Chemistry
ISSN/ISBN 1463-9076 ; 1463-9084
edoc-URL http://edoc.unibas.ch/dok/A6002729
Full Text on edoc No
Digital Object Identifier DOI 10.1039/c2cp23872a
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/22402636
ISI-Number WOS:000301957900024
Document type (ISI) Journal Article

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