Artificial enzymes made to order : combination of computational design and directed evolution

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Artificial enzymes made to order : combination of computational design and directed evolution

JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)

ID	116784
Author(s)	Ward, Thomas R.
Author(s) at UniBasel	Ward, Thomas R.
Year	2008
Title	Artificial enzymes made to order : combination of computational design and directed evolution
Journal	Angewandte Chemie International Edition
Volume	47
Number	41
Pages / Article-Number	7802-3
Keywords	artificial enzymes, directed evolution, elimination, enzyme design, proton transfer
Abstract	Intelligent design: The combination of computational design and directed evolution has allowed the creation and optimization of an artificial enzyme starting from a catalytically inactive protein scaffold. In this approach, glutamate, tryptophane, and lysine residues were introduced into a TIM barrel fold to yield an artificial enzyme for the Kemp elimination that was fine-tuned by directed evolution (kcat/Km = 2590 M-1 S-1).
Publisher	Wiley
ISSN/ISBN	1433-7851 ; 1521-3773
edoc-URL	http://edoc.unibas.ch/dok/A5254499
Full Text on edoc	No
Digital Object Identifier DOI	10.1002/anie.200802865
PubMed ID	http://www.ncbi.nlm.nih.gov/pubmed/18780363
ISI-Number	WOS:000260062500003
Document type (ISI)	Journal Article

13/05/2024

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