Artificial enzymes made to order : combination of computational design and directed evolution
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 116784
Author(s) Ward, Thomas R.
Author(s) at UniBasel Ward, Thomas R.
Year 2008
Title Artificial enzymes made to order : combination of computational design and directed evolution
Journal Angewandte Chemie International Edition
Volume 47
Number 41
Pages / Article-Number 7802-3
Keywords artificial enzymes, directed evolution, elimination, enzyme design, proton transfer
Abstract Intelligent design: The combination of computational design and directed evolution has allowed the creation and optimization of an artificial enzyme starting from a catalytically inactive protein scaffold. In this approach, glutamate, tryptophane, and lysine residues were introduced into a TIM barrel fold to yield an artificial enzyme for the Kemp elimination that was fine-tuned by directed evolution (kcat/Km = 2590 M-1 S-1).
Publisher Wiley
ISSN/ISBN 1433-7851 ; 1521-3773
edoc-URL http://edoc.unibas.ch/dok/A5254499
Full Text on edoc No
Digital Object Identifier DOI 10.1002/anie.200802865
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/18780363
ISI-Number WOS:000260062500003
Document type (ISI) Journal Article
 
   

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