(Strept)avidin as Host for Biotinylated Coordination Complexes:  Stability, Chiral Discrimination, and Cooperativity
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 116744
Author(s) Loosli, Andreas; Rusbandi, Untung Edy; Gradinaru, Julieta; Bernauer, Klaus; Schlaepfer, Carl Wilhelm; Meyer, Michel; Mazurek, Sylwester; Novic, Marjana; Ward, Thomas R.
Author(s) at UniBasel Ward, Thomas R.
Year 2006
Title (Strept)avidin as Host for Biotinylated Coordination Complexes:  Stability, Chiral Discrimination, and Cooperativity
Journal Inorganic Chemistry
Volume 45
Number 2
Pages / Article-Number 660-8
Keywords Avidins Role: PEP (Physical, engineering or chemical process), PRP (Properties), PYP (Physical process), PROC (Process) (assocn. consts. with ruthenium bipyridine biotinylated enantiomeric complexes); Formation constant (assocn.; of ruthenium bipyridine biotinylated enantiomeric complexes with avidin and streptavidin); stability const ruthenium bipyridine biotinylated complex avidin streptavidin; ruthenium bipyridine biotinylated complex prepn assocn avidin streptavidin
Abstract Incorporation of a biotinylated ruthenium tris(bipyridine) [Ru(bpy)2(Biot-bpy)]2+ (1) in either avidin or streptavidin(strept)avidincan be conveniently followed by circular dichroism spectroscopy. To determine the stepwise association constants, cooperativity, and chiral discrimination properties, diastereopure (Λ and Δ)-1 species were synthesized and incorporated in tetrameric (strept)avidin to afford (Δ-[Ru(bpy)2(Biot-bpy)]2+)x⊂avidin, (Λ-[Ru(bpy)2(Biot-bpy)]2+)x⊂avidin, (Δ-[Ru(bpy)2(Biot-bpy)]2+)x⊂streptavidin, and (Λ-[Ru(bpy)2(Biot-bpy)]2+)x⊂streptavidin (x = 1−4) For these four systems, the overall stability constants are log β4 = 28.6, 30.3, 36.2, and 36.4, respectively. Critical analysis of the CD titrations data suggests a strong cooperativity between the first and the second binding event (x = 1, 2) and a pronounced difference in affinity between avidin and streptavidin for the dicationic guest 1 as well as modest enantiodiscrimination properties with avidin as host.
Publisher American Chemical Society
ISSN/ISBN 0020-1669 ; 1520-510X
edoc-URL http://edoc.unibas.ch/dok/A5254460
Full Text on edoc No
Digital Object Identifier DOI 10.1021/ic051405t
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/16411701
Document type (ISI) Journal Article

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