Acetylation regulates the DNA end-trimming activity of DNA polymerase beta
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 110601
Author(s) Hasan, Sameez; El-Andaloussi, Nazim; Hardeland, Ulrike; Hassa, Paul O; Bürki, Christine; Imhof, Ralph; Schär, Primo; Hottiger, Michael O
Author(s) at UniBasel Schär, Primo Leo
Year 2002
Title Acetylation regulates the DNA end-trimming activity of DNA polymerase beta
Journal Molecular cell
Volume 10
Number 5
Pages / Article-Number 1213-22

We describe a novel regulatory mechanism for DNA polymerase beta (Polbeta), a protein involved in DNA base excision repair (BER). Polbeta colocalized in vivo and formed a complex with the transcriptional coactivator p300. p300 interacted with Polbeta through distinct domains and acetylated Polbeta in vitro. Polbeta acetylation was furthermore observed in vivo. Lysine 72 of Polbeta was identified as the main target for acetylation by p300. Interestingly, acetylated Polbeta showed a severely reduced ability to participate in a reconstituted BER assay. This was due to an impairment of the dRP-lyase activity of Polbeta. Acetylation of Polbeta thus acts as an intranuclear regulatory mechanism and implies that p300 plays a critical regulatory role in BER.

Publisher Cell Press
ISSN/ISBN 1097-2765
Full Text on edoc No
Digital Object Identifier DOI 10.1016/S1097-2765(02)00745-1
PubMed ID
ISI-Number WOS:000179450600027
Document type (ISI) Journal Article

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