β-Amyloid protein amplifies calcium signalling in central neurons from the adult mouse
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 107263
Author(s) Hartmann, H; Eckert, A; Müller, W E
Author(s) at UniBasel Eckert, Anne
Year 1993
Title β-Amyloid protein amplifies calcium signalling in central neurons from the adult mouse
Journal Biochemical and Biophysical Research Communications
Volume 194
Number 3
Pages / Article-Number 1216-20
Abstract

The role of beta-amyloid in Alzheimer's disease and its cellular mechanism of action are still unclear. Based on observations that beta-amyloid influences neuronal calcium homeostasis we investigated the effect of the peptide on K(+)-induced enhancement of free intracellular calcium in dissociated neurons from adult mice. Preincubation with beta-amyloid fragment 25-35 at concentrations > or = 0.05 mumol/l resulted in marked amplification of the K(+)-induced Ca2+ response. This effect was also observed with fragment 1-40, whereas fragment 1-28 or 12-28 did not affect the Ca2+ response. This preparation therefore presents a valuable model to investigate the action of beta-amyloid ex vivo in individual animals. Our findings suggest a small but consisting destabilizating effect of beta-amyloid on neuronal Ca2+ homeostasis resulting in chronically increased neuronal vulnerability.

Publisher Elsevier
ISSN/ISBN 0006-291X
edoc-URL http://edoc.unibas.ch/dok/A5253525
Full Text on edoc No
Digital Object Identifier DOI 10.1006/bbrc.1993.1952
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/8352778
ISI-Number WOS:A1993LT51700035
Document type (ISI) Journal Article
 
   

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