Ancylostoma caninum : calibration and comparison of diagnostic accuracy of flotation in tube, McMaster and FLOTAC in faecal samples of dogs
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1022818
Author(s) Cringoli, Giuseppe; Rinaldi, Laura; Maurelli, Maria Paola; Morgoglione, Maria Elena; Musella, Vincenzo; Utzinger, Jürg
Author(s) at UniBasel Utzinger, Jürg
Year 2011
Title Ancylostoma caninum : calibration and comparison of diagnostic accuracy of flotation in tube, McMaster and FLOTAC in faecal samples of dogs
Journal Experimental parasitology : EP
Volume 128
Number 1
Pages / Article-Number 32-7
Keywords Ancylostoma caninum, Dog, Diagnosis, FLOTAC, Flotation in tube, McMaster
Abstract We performed a calibration of flotation in tube, McMaster and FLOTAC to determine the optimal flotation solution (FS) and the influence of faecal preservation for the diagnosis of Ancylostoma caninum in dogs, and compared the accuracy of the three copromicroscopic techniques. Among nine different FS, sodium chloride and sodium nitrate performed best for detection and quantification of A.caninum eggs. Faecal samples, either fresh or preserved in formalin 5%, resulted in higher A.caninum egg counts, compared to frozen samples or preserved in formalin 10% or sodium acetate-acetic acid-formalin. FLOTAC consistently resulted in higher A.caninum eggs per gram of faeces (EPG) and lower coefficient of variation (CV) than McMaster and flotation in tube. The best results in terms of mean faecal egg counts (highest value, i.e. 117.0 EPG) and CV (lowest value, i.e. 4.8%) were obtained with FLOTAC using sodium chloride and faecal samples preserved in formalin 5%. Our findings suggest that the FLOTAC technique should be considered for the diagnosis of A.caninum in dogs
Publisher Academic Press
ISSN/ISBN 0014-4894
edoc-URL http://edoc.unibas.ch/dok/A6002106
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.exppara.2011.01.014
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/21295030
ISI-Number WOS:000289034700005
Document type (ISI) Journal Article
 
   

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