Absorbance detector for high-performance liquid chromatography based on light-emitting diodes for the deep-ultraviolet range
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1007369
Author(s) Bomastyk, Benjamin; Petrovic, Igor; Hauser, Peter C
Author(s) at UniBasel Hauser, Peter C.
Bomastyk, Benjamin
Year 2011
Title Absorbance detector for high-performance liquid chromatography based on light-emitting diodes for the deep-ultraviolet range
Journal Journal of chromatography A
Volume 1218
Number 24
Pages / Article-Number 3750-6
Keywords HPLC, Absorption detection, Deep-UV, Light-emitting diode
Abstract

A HPLC-detector has been designed which employs light-emitting diodes in the deep-UV-range below 300 nm as wavelength specific radiation sources and special UV-photodiodes for measuring the signal. A monochromator is therefore not needed. The design features a beam splitter and a reference photodiode, precision mechanics for adjustment of the light beams and electronics for stabilization of the LED-current. The processing of the photodiode currents is carried out with a high performance log-ratio amplifier which allows direct absorbance measurements. The optical and electronic performance of the detector was characterised and high precision over several absorbance units was obtained. Testing of analytical separation methods in isocratic as well as gradient modes employing UV-detection at 255 and 280 nm showed a very similar performance to a commercial photodiode-array detector used in the fixed wavelength mode in terms of linearity, precision and detection limits. The chief advantages of the new device are small size, low power consumption, and low cost. (C) 2011 Elsevier B.V. All rights reserved.

Publisher Elsevier
ISSN/ISBN 0021-9673
edoc-URL http://edoc.unibas.ch/dok/A6001932
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.chroma.2011.04.039
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/21531417
ISI-Number WOS:000291576400004
Document type (ISI) Journal Article
 
   

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